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human thymus cdna library  (TaKaRa)


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    Structured Review

    TaKaRa human thymus cdna library
    Human Thymus Cdna Library, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+thymus+matchmaker+cdna+library/Human+Thymus+Matchmaker+cDNA+Library+pACT2/pmc02825663-56-1-12
    Average 93 stars, based on 16 article reviews
    human thymus cdna library - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    cDNA Library Assay:

    Article Title: DAXX Is a New AIRE-interacting Protein
    Article Snippet: Two-hybrid Assay Screens Yeast two-hybrid assays were performed using GAL4 Matchmaker Two-hybrid System 3 (Clontech). .. A human thymus Matchmaker cDNA library in the pACT2 prey plasmid (Clontech) was screened for proteins that interact with AIRE (aa 1–517) in the pGBKT7-DNA-BD bait plasmid using the AH109 yeast reporter strain. ..

    Article Title: Molecular basis of actin reorganization promoted by binding of enterohaemorrhagic Escherichia coli EspB to alpha-catenin.
    Article Snippet: .. The cDNA clone of a-catenin was kindly provided by M. Ozawa (Graduate School of Medical and Dental Sciences, Kagoshima University, Japan), and the clone of b-catenin was obtained from a human thymus Matchmaker cDNA library (Clontech, Palo Alto, CA). .. These proteins were expressed using E. coli BL21(DE3) transformed with an expression vector produced by ligating the coding sequence of human a-catenin digested with NdeI and SalI, or the sequence of b-catenin digested with BamHI and SalI, into the multicloning site of pET28a (Novagen, Madison, WI, USA).

    Article Title: Length-dependent Binding of Human XLF to DNA and Stimulation of XRCC4·DNA Ligase IV Activity
    Article Snippet: .. Construction of GST-XLF and XLF-Myc-His-Expressing Plasmids—Full-length human XLF cDNA was amplified by recombinant Pfu DNA polymerase (Stratagene, catalog #600154) using a human thymus matchmaker cDNA library (Clontech, catalog #HL4057AH) as the template (primers are available upon request). ..

    Article Title: HAT cofactor TRRAP mediates beta-catenin ubiquitination on the chromatin and the regulation of the canonical Wnt pathway.
    Article Snippet: .. The Two Hybrid screen was performed with the Human Thymus Matchmaker cDNA library (Clontech, Pont de Claix, France). ..

    Article Title: DAXX Is a New AIRE-interacting Protein
    Article Snippet: Yeast two-hybrid assays were performed using GAL4 Matchmaker Two-hybrid System 3 (Clontech). .. A human thymus Matchmaker cDNA library in the pACT2 prey plasmid (Clontech) was screened for proteins that interact with AIRE (aa 1–517) in the pGBKT7-DNA-BD bait plasmid using the AH109 yeast reporter strain. ..

    Plasmid Preparation:

    Article Title: DAXX Is a New AIRE-interacting Protein
    Article Snippet: Two-hybrid Assay Screens Yeast two-hybrid assays were performed using GAL4 Matchmaker Two-hybrid System 3 (Clontech). .. A human thymus Matchmaker cDNA library in the pACT2 prey plasmid (Clontech) was screened for proteins that interact with AIRE (aa 1–517) in the pGBKT7-DNA-BD bait plasmid using the AH109 yeast reporter strain. ..

    Article Title: DAXX Is a New AIRE-interacting Protein
    Article Snippet: Yeast two-hybrid assays were performed using GAL4 Matchmaker Two-hybrid System 3 (Clontech). .. A human thymus Matchmaker cDNA library in the pACT2 prey plasmid (Clontech) was screened for proteins that interact with AIRE (aa 1–517) in the pGBKT7-DNA-BD bait plasmid using the AH109 yeast reporter strain. ..

    Amplification:

    Article Title: Length-dependent Binding of Human XLF to DNA and Stimulation of XRCC4·DNA Ligase IV Activity
    Article Snippet: .. Construction of GST-XLF and XLF-Myc-His-Expressing Plasmids—Full-length human XLF cDNA was amplified by recombinant Pfu DNA polymerase (Stratagene, catalog #600154) using a human thymus matchmaker cDNA library (Clontech, catalog #HL4057AH) as the template (primers are available upon request). ..

    Recombinant:

    Article Title: Length-dependent Binding of Human XLF to DNA and Stimulation of XRCC4·DNA Ligase IV Activity
    Article Snippet: .. Construction of GST-XLF and XLF-Myc-His-Expressing Plasmids—Full-length human XLF cDNA was amplified by recombinant Pfu DNA polymerase (Stratagene, catalog #600154) using a human thymus matchmaker cDNA library (Clontech, catalog #HL4057AH) as the template (primers are available upon request). ..

    Two Hybrid Screening:

    Article Title: HAT cofactor TRRAP mediates beta-catenin ubiquitination on the chromatin and the regulation of the canonical Wnt pathway.
    Article Snippet: .. The Two Hybrid screen was performed with the Human Thymus Matchmaker cDNA library (Clontech, Pont de Claix, France). ..



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